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Interaction between clenbuterol and run training: effects on exercise performance and MLC isoform content
C P Ingalls1, W S Barnes, S B Smith
1Department of Health, Texas A&M University, College Station 77843, USA.
Clenbuterol (CB) impairs running performance, while exercise training enhances it. Combining CB and exercise negates performance benefits but preserves CB
Area of Science:
- Exercise Physiology
- Skeletal Muscle Biology
- Pharmacology
Background:
- Clenbuterol (CB) is known for its anabolic effects on skeletal muscle.
- The impact of CB on exercise performance and its interaction with training remains unclear.
- Myosin light-chain (MLC) isoform expression is critical for muscle function and adaptation.
Purpose of the Study:
- To investigate the individual and combined effects of clenbuterol (CB) and interval training on running performance.
- To examine the impact of CB and exercise on myosin light-chain (MLC) isoform expression in mouse skeletal muscle.
- To determine if CB administration influences exercise-induced adaptations in skeletal muscle.
Main Methods:
- Mice were divided into four groups: control, exercise (Ex), clenbuterol (CB), and exercise + clenbuterol (Ex + CB).
- Mice received CB orally or placebo, and underwent treadmill interval training for 8 weeks.
- Running performance was assessed via a run-to-exhaustion test, and skeletal muscle MLC isoforms were analyzed using polyacrylamide gel electrophoresis.
Main Results:
- Exercise training significantly increased total work performed, while CB administration decreased it.
- The combination of CB and exercise eliminated the performance gains from training, showing antagonistic effects on running performance.
- Neither CB nor exercise training, alone or combined, altered MLC isoform expression in key leg muscles.
- Both CB and Ex + CB groups showed significant increases in muscle mass, total protein, and myofibrillar protein compared to controls.
Conclusions:
- Clenbuterol (CB) administration negatively impacts running performance.
- The combination of exercise and CB results in antagonistic effects on running performance.
- CB's anabolic effects on skeletal muscle mass and protein content are not diminished by exercise training.
- Neither CB nor exercise training alters myosin light-chain (MLC) isoform profiles in mouse skeletal muscle.
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