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[Evaluation of methods of sperm preparation for human in vitro fertilization]
Objectives:
Compare results of sperm prepared with centrifiguation-migration, percoll discontinuous gradient (50, 70, 90%) and migration-sedimentation.
Methods:
An aliquot of sperm collected from 125 men referred for infertility was prepared according to the three methods. The spermogram after preparation and extration rates were compared using the Student's t test for paired variables and for three subpopulations identified by increasing spermatozoid concentrations.
Results:
Sperm count were significantly higher after percoll. Mobility and morphology were improved after centrifugation-migration excepting for the samples with the lowest counts. The extration rate was better after percoll and rose for the low-count samples.
Conclusion:
Percoll gradient is the most effective method, particularly for altered sperm. The number of mobile and normal forms can be improved with the centrifugation-migration technique.