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[Current diagnosis, clinical course and treatment of acute colitis infection with Clostridium difficile]
1Kliniki Obserwacyjno-Zakaźnej AM w Białymstoku.
Abstract:
Detection rate of G. intestionalis in feces with direct microscopy has been compared with the immuno-enzymatic technique detecting protein GSA 65 with Alexon Inc., ProSpec T/Giardia reagents kit. The results obtained with both methods have further been compared with those obtained by microscopic examination of the duodenal content. Detectability of Giardia intestinalis with EIA technique with the use of ready-made kit has been assessed. Feces have been collected from 371 patients. Protein GSA 65 has been present in 170 samples, 45.8%, examined with the use of ProSpec T/Gardia kit. Giardia intestinalis cysts have been detected microscopically in the feces of 37 patients, i.e. in 22.3%. Microscopic examinations carried out by three independent examiners have shown marked diversity in the rate of positive results, being 0.1% (examiner A), 28.6% (B), and 45.2% (C). Comparison of G. intestinale detectability of all 3 techniques used have shown absence of protein GSA 65 in 2 out of 9 examined patients. However, trophozoits have been present in the duodenal content. Test performed with kits made by Alexon Inc. and DIALAB have given 45.8% and 60.7% of the positive results, respectively.
Insights
The immuno-enzymatic technique for detecting Giardia intestinalis (G. intestinalis) protein GSA 65 in feces showed higher detection rates compared to direct microscopy. Microscopic examination of duodenal content also revealed discrepancies in G. intestinalis detection.
Area of Science:
- Medical Parasitology
- Diagnostic Microbiology
- Gastroenterology
Background:
- Giardia intestinalis (G. intestinalis) is a common cause of parasitic infections.
- Accurate detection of G. intestinalis is crucial for effective treatment.
- Direct microscopy for G. intestinalis detection in feces can be unreliable.
Purpose of the Study:
- To compare the detection rates of G. intestinalis using direct microscopy versus an immuno-enzymatic technique (EIA) detecting GSA 65.
- To evaluate the efficacy of a ready-made EIA kit for G. intestinalis detection.
- To compare these methods with microscopic examination of duodenal content.
Main Methods:
- Fecal samples from 371 patients were analyzed.
- Direct microscopy of feces was performed by three independent examiners.
- An immuno-enzymatic assay (EIA) using ProSpec T/Giardia reagents kit (Alexon Inc.) to detect GSA 65 protein was employed.
- Microscopic examination of duodenal content was also conducted.
Main Results:
- The EIA kit detected GSA 65 protein in 45.8% of fecal samples.
- Direct microscopy detected G. intestinalis cysts in only 22.3% of fecal samples.
- Microscopic examination of feces showed significant variability among examiners (0.1% to 45.2%).
- EIA kits from Alexon Inc. and DIALAB yielded positive results in 45.8% and 60.7% of cases, respectively.
- Two patients negative for GSA 65 in feces were positive for trophozoites in duodenal content.
Conclusions:
- The immuno-enzymatic technique (EIA) demonstrates a higher detection rate for Giardia intestinalis (G. intestinalis) compared to direct fecal microscopy.
- Significant inter-observer variability exists in microscopic diagnosis of G. intestinalis.
- Ready-made EIA kits offer a potentially more reliable diagnostic approach for G. intestinalis infections.