[The effect of cytoskeleton modification on the process of trypsin-induced cell aggregation]
Related Experiment Video
Updated: Nov 3, 2026

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System
Published on: May 22, 2020
Abstract:
Studies were performed on the influence of cytoskeleton modification (by colchicine, vinblastine, and cytochalasin B, and by neoplastic transformation) on cell aggregation induced by small trypsin concentrations (the final concentrations equal to 1-100 mg/ml) added to a suspension of chick embryo fibroblasts dispersed from a monolayer with EDTA. It was shown that alteration of microfilaments resulted in the loss of the ability of plasma membranes to structural rearrangements. It is suggested that aggregation of these cells is realized due to a gradual exposure of adhesive sites along with proteolytic splitting of protein components of plasma membranes.
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