Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antioxidant enzyme expression in rat lungs during hyperoxia

Y S Ho1, M S Dey, J D Crapo

  • 1Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA.

The American Journal of Physiology
|May 1, 1996
PubMed
Summary

High oxygen exposure significantly increases manganese-containing superoxide dismutase (MnSOD) mRNA in rat lungs. However, MnSOD enzyme activity does not proportionally increase, suggesting post-transcriptional regulation of antioxidant defenses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Urgent need to establish an official Chinese medicine information dissemination platform in Hong Kong to cope with the outbreak of infectious diseases.

Hong Kong medical journal = Xianggang yi xue za zhi·2025
Same author

Response to letter to the editor : Trends of impact factor contributors to the Injury Journal: A bibliometric analysis.

Injury·2025
Same author

Effects of electroacupuncture on postoperative cognitive dysfunction: a preclinical study (abridged secondary publication).

Hong Kong medical journal = Xianggang yi xue za zhi·2022
Same author

Neuroprotective effects of oxyresveratrol on 6-hydroxydopamine on medial forebrain bundles in a rat model of Parkinson disease: abridged secondary publication.

Hong Kong medical journal = Xianggang yi xue za zhi·2020
Same author

Asthma in children: mapping the literature by bibliometric analysis.

Revue francaise d'allergologie et d'immunologie clinique·2020
Same author

Mitochondrial uncoupling reveals a novel therapeutic opportunity for p53-defective cancers.

Nature communications·2018

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pulmonary Medicine

Background:

  • Pulmonary antioxidant enzymes protect lungs from oxidative stress.
  • Hyperoxia (high oxygen exposure) can induce oxidative damage.
  • Understanding antioxidant enzyme regulation is crucial for lung health.

Purpose of the Study:

  • Investigate molecular mechanisms of pulmonary antioxidant enzyme upregulation during hyperoxia.
  • Determine mRNA and enzyme activity levels of key antioxidant enzymes in rats exposed to high oxygen.

Main Methods:

  • Exposure of adult rats to 85% oxygen.
  • Quantification of lung mRNA for various antioxidant enzymes using RT-PCR.
  • Measurement of enzyme activities (CuZnSOD, MnSOD) and protein/DNA content.

Related Experiment Videos

  • Nuclear run-on assays to assess gene transcription rates.

    • Main Results:

    • Hyperoxia significantly induced manganese-containing superoxide dismutase (MnSOD) mRNA (up to 600% increase).
    • Transcriptional rate of MnSOD gene increased by ~400% at day 3.
    • MnSOD enzyme activity did not proportionally increase with mRNA levels, suggesting post-transcriptional regulation.

    Conclusions:

    • Transcriptional activation plays a role in MnSOD gene upregulation during hyperoxia.
    • Translational and/or post-translational regulation are critical for controlling lung MnSOD activity under high oxygen conditions.