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Multiplex PCR for diagnosis of AIDS-related central nervous system lymphoma and toxoplasmosis
1Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Journal of Clinical Microbiology
|January 1, 1997
Abstract:
A nested multiplex PCR assay was designed for the simultaneous detection of Epstein-Barr virus and Toxoplasma gondii DNA from the cerebrospinal fluid of AIDS patients. T. gondii DNA was detected in 8 of 8 patients with Toxoplasma encephalitis and in 0 of 6 patients without toxoplasmosis, and Epstein-Barr virus DNA was found in 9 of 14 patients with central nervous system lymphoma and in 2 of 38 patients without disease.