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Molecular interaction between limb deformity proteins (formins) and Src family kinases
1EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany. Zeller@EMBL-Heidelberg.de
The Journal of Biological Chemistry
|December 27, 1996
Summary
Limb deformity (Ld) proteins, crucial for embryonic polarity, directly bind to Src family kinases. This interaction occurs in the nucleus and at cellular membranes, linking Ld proteins to signal transduction pathways.
Area of Science:
- Cell Biology
- Molecular Biology
- Developmental Biology
Background:
- Limb deformity (Ld) proteins, encoded by the limb deformity (ld) gene, are key regulators of embryonic polarity.
- Formins, including Ld proteins, are involved in diverse cellular processes.
Purpose of the Study:
- To investigate the interaction between Ld proteins and Src family kinases.
- To elucidate the molecular mechanisms and cellular localization of this interaction.
Main Methods:
- Co-immunoprecipitation assays to confirm in vivo association.
- Biochemical fractionation and immunolocalization to determine protein localization.
- Analysis of protein domains involved in binding (proline-rich domain, SH3 domain).
Main Results:
- Chicken and murine Ld proteins directly interact with Src family kinases (c-Src and c-Fyn).
- Binding is mediated by the Ld protein's proline-rich domain and the Src SH3 domain.
- Ld proteins, predominantly nuclear, also associate with cellular membranes, co-localizing with c-Src.
- Association occurs at both plasma and perinuclear membranes.
Conclusions:
- Ld proteins interact with Src family kinases, suggesting a role in signal transduction.
- The interaction occurs both in the nucleus and at cellular membranes.
- This association links morphoregulatory Ld proteins to membrane-bound kinase signaling pathways.
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