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Approaches to detection of distantly related proteins by database searches
K Cattell1, B Koop, R S Olafson
1University of Victoria, BC, Canada.
Biotechniques
|December 1, 1996
Abstract:
The searching of protein databases as a method of identifying newly sequenced genes is commonplace in molecular biology laboratories. However, it is a procedure that is not usually formally taught to students, and method cookbooks discuss it only briefly. This article uses a single family of highly diverged uracil-DNA glycosylases, which fall into two distinct groups, to highlight some of the difficulties associated with identification of such proteins by database searching.