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Related Experiment Videos

Corticosteroid synthesis in the central nervous system

C E Gomez-Sanchez1, M Y Zhou, E N Cozza

  • 1Department of Internal Medicine, Harry S. Truman Memorial Veterans Hospital, Columbia, Missouri, USA.

Endocrine Research
|November 1, 1996
PubMed
Summary

Central nervous system tissues can produce adrenocorticosteroids, indicated by the presence of 11 beta-hydroxylase (CYP11B1) mRNA and steroid conversion. This suggests a potential role in blood pressure regulation.

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Area of Science:

  • Neuroendocrinology
  • Molecular Biology
  • Physiology

Background:

  • Adrenocorticosteroids are crucial hormones primarily synthesized in the adrenal glands.
  • The central nervous system's (CNS) potential role in synthesizing these steroids remains largely unexplored.
  • 11 beta-hydroxylase (CYP11B1) is a key enzyme in corticosterone and cortisol production.

Purpose of the Study:

  • To investigate the potential synthesis of adrenocorticosteroids within the central nervous system.
  • To identify the presence and function of the 11 beta-hydroxylase enzyme in brain tissues.
  • To assess the impact of inhibiting this enzyme on salt-induced hypertension in rats.

Main Methods:

  • RT-PCR was used to detect CYP11B1 mRNA in various brain regions and the aorta.

Related Experiment Videos

  • Brain tissue minces were incubated with radiolabeled DOC to measure steroid metabolite formation.
  • The effect of intracerebroventricular infusion of an 11 beta-hydroxylase inhibitor (19-ethynyldeoxycorticosterone) on blood pressure was evaluated.

    • Main Results:

    • Significant levels of CYP11B1 mRNA were detected in the aorta, cerebrum, cerebellum, hippocampus, hypothalamus, and amygdala, but not the heart.
    • Brain tissue minces demonstrated conversion of 3H-DOC to corticosterone and 11-dehydrocorticosterone.
    • The inhibitor's effect on salt-induced hypertension was dose-dependent, with lower doses preventing hypertension and higher doses having no effect or increasing it.

    Conclusions:

    • The central nervous system possesses the capacity for adrenocorticosteroid synthesis, evidenced by CYP11B1 expression and steroidogenic activity.
    • The presence of 11 beta-hydroxylase in brain tissues suggests a localized role for these steroids.
    • Inhibition of CNS 11 beta-hydroxylase influences salt-induced hypertension, highlighting its potential involvement in blood pressure regulation.