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A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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A method for introducing site-specific mutations using oligonucleotide primers and its application to site-saturation

M J O'Donohue1, G G Kneale

  • 1Laboratoire de Médiateurs Protéiques, INRA Centre de Jouy-en-Josas, France.

Molecular Biotechnology
|October 1, 1996
PubMed
Summary

Oligonucleotide primer-directed mutagenesis enables protein structure-function studies and the creation of novel mutant proteins. This method is crucial for site-saturation mutagenesis, generating diverse amino acid substitutions efficiently.

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