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Streptomyces linear plasmids that contain a phage-like, centrally located, replication origin

P C Chang1, E S Kim, S N Cohen

  • 1Department of Genetics, Stanford University School of Medicine, California 94305, USA.

Molecular Microbiology
|December 1, 1996
PubMed
Summary

The Streptomyces plasmid pSLA2 uses an internal origin of replication, distinct from telomeric initiation. This origin contains direct repeats and genes for essential proteins Rep1 and Rep2, suggesting unique replication mechanisms.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Linear plasmids in Streptomyces, such as pSLA2, exhibit unique replication strategies.
  • Unlike typical linear replicons, pSLA2 initiates replication internally, not at telomeres.

Purpose of the Study:

  • To identify and characterize the replication origin of the Streptomyces plasmid pSLA2.
  • To investigate the genetic elements and proteins involved in pSLA2 replication.

Main Methods:

  • Identification of direct repeats (iterons) within the pSLA2 replication origin.
  • Characterization of genes encoding essential DNA-binding protein (Rep1) and a DNA helicase-like protein (Rep2).
  • Analysis of a separate locus essential for replication in cis.

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Main Results:

  • The pSLA2 replication origin contains direct repeats (iterons) within the Rep1 gene.
  • Essential proteins Rep1 and Rep2 are co-expressed from the same transcript.
  • A distinct 430 bp locus is required in cis for plasmid replication.
  • Similar replication configurations were observed in the Streptomyces clavuligerus plasmid pSCL.

Conclusions:

  • Streptomyces linear plasmids possess internal replication origins with unique genetic components.
  • The findings suggest Streptomyces linear plasmids represent an evolutionary link between circular plasmids and linear phage replicons.