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Related Experiment Videos

Soluble P-selectin as parameter for platelet activation during storage

E H Kostelijk1, R Fijnheer, H K Nieuwenhuis

  • 1Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands.

Thrombosis and Haemostasis
|December 1, 1996
PubMed
Summary
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Platelet concentrates deteriorate during storage. Soluble P-selectin detection effectively quantifies early platelet activation, offering a more sensitive assay than traditional methods.

Area of Science:

  • Transfusion Medicine
  • Hematology
  • Biochemistry

Background:

  • Platelet concentrates stored at room temperature undergo deterioration, known as storage lesion.
  • This lesion involves morphological changes and functional decline in platelets.

Purpose of the Study:

  • To identify a reliable assay for detecting early platelet activation in stored platelet concentrates.
  • To compare soluble P-selectin levels with established markers of platelet activation.

Main Methods:

  • Quantification of soluble P-selectin using ELISA in storage medium.
  • Assessment of morphological score and beta-thromboglobulin (beta-TG) release.
  • Flow-cytometric analysis of P-selectin expression on platelets.

Main Results:

Related Experiment Videos

  • Significant correlations were found between soluble P-selectin and P-selectin-positive platelets (r=0.7449) and beta-TG release (r=0.6837).
  • A strong negative correlation existed between soluble P-selectin and morphological score (r=-0.7669).
  • Soluble P-selectin levels increased consistently throughout storage from day 0 to day 8.

Conclusions:

  • Soluble P-selectin detection is a sensitive method for quantifying platelet activation during storage.
  • This immunoassay allows for earlier detection of platelet activation compared to flow-cytometry.