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Updated: Aug 9, 2026

Isolation and Activation of Murine Lymphocytes
Published on: October 30, 2016
Glutamine, lymphocyte proliferation and cytokine production
T Rohde1, D A MacLean, B Klarlund Pedersen
1Copenhagen Muscle Research Centre, Department of Infectious Diseases, Rigshospitalet, University Hospital, Denmark.
Abstract:
The present in vitro study was conducted to examine how glutamine influences the lymphocyte function. Glutamine had no effect on the production of interleukin-1 beta, interleukin-6 or tumour necrosis factor-alpha, but influenced the production of interleukin-2 and interferon-gamma. Glutamate, leucine, isoleucine and valine (substrates for glutamine production), or the combination of glutamate and leucine, did not influence the lymphocyte proliferative response or the cytokine production. In conclusion, glutamine influenced the production of some T-cell-derived cytokines, and is thereby important for optimal lymphocyte proliferation. Furthermore, the results show that lymphocytes are not capable of producing glutamine.
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