3H-delta9-tetrahydrocannabinol distribution in pregnant dogs and their fetuses

Pregnant dogs were administered intravenously 0.5 mg/kg of 3H-delta9-tetrahydrocannabinol and sacrificed at 30 minutes, the time of peak behavioral effects. The distribution of radioactivity in mothers and fetuses was quantified in many peripheral tissues and in major brain areas by sample combustion and liquid scintillation spectrometry. Brains were subfractionated by differential centrifugation in order to compare the subcellular localization of radioactivity in maternal and fetal brains. Although the level of radioactivity in maternal brain was three times higher than that in fetal brain, the distribution of radioactivity in the major brain areas was similar in mothers and fetuses. However, the subcellular distribution of radioactivity in whole brain homogenate of fetal dogs was markedly different from that in whole brain homogenates from mothers and appeared to be related directly to differing phospholipid content.