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Related Experiment Videos

Subcellular localization of lead in synaptosomes

E K Silbergeld, H S Adler, J L Costa

    Research Communications in Chemical Pathology and Pharmacology
    |August 1, 1977
    PubMed
    Summary

    Lead neurotoxicity may stem from its accumulation in nerve terminal mitochondria. This study found lead and associated elements within mitochondria of isolated synaptosomes, suggesting a primary site of action for lead poisoning.

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    Area of Science:

    • Neuroscience
    • Toxicology
    • Cell Biology

    Background:

    • Lead neurotoxicity impacts cholinergic and monoaminergic pathways.
    • The precise site of lead's neurotoxic action remains unknown.

    Purpose of the Study:

    • To investigate the in vitro interaction of lead with synaptosomes.
    • To identify the subcellular location of lead within nerve terminals.

    Main Methods:

    • X-ray elemental analysis coupled scanning-transmission electron microscopy (STEM-XRE) was used.
    • Isolated synaptosomes were air-dried on EM grids without fixation or staining to prevent element translocation.
    • Synaptosomes and intrasynaptosomal mitochondria were analyzed for lead distribution.

    Main Results:

    • Lead was exclusively detected within intrasynaptosomal mitochondria.
    • Lead accumulation in mitochondria was associated with calcium (Ca) and phosphate (P).
    • Synaptosomes and their mitochondria were clearly distinguishable in the prepared samples.

    Conclusions:

    • Lead enters isolated nerve terminals (synaptosomes).
    • Lead is sequestered within mitochondria in nerve terminals.
    • Mitochondrial accumulation of lead may be a key factor in its neurotoxicity.

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