Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Statistical methods for gene map construction by fluorescence in situ hybridization

S W Guo1, W L Flejter

  • 1Department of Biostatistics, School of Public Health, University of Michigan, Ann Arbor 48109-2029, USA. swguo@sph.umich.edu

Genome Research
|December 1, 1996
PubMed
Summary

Bayesian methods enhance fluorescence in situ hybridization (FISH) for accurate locus ordering on chromosomes. This approach improves the reliability of gene mapping by calculating the probability of correct locus order.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Research on the evaluation of the automatic construction effectiveness of a flexible point cloud deformation algorithm for nasal prostheses].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2026
Same author

[Analysis of adjuvant chemotherapy efficacy based on GLUT1 expression levels after radical resection of pancreatic cancer].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2026
Same author

[Study on the consistency between the trajectory of small opening and closing movements and the empirical hinge axis movement of the mandibular incisal point of healthy individuals].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2026
Same author

[Clinical value of secondary surgical resection for recurrent or metastatic pancreatic cancer after initial surgery].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2026
Same author

[Arterial prophylactic occlusion technique in the application of surgery for locally advanced pancreatic cancer with arterial involvement after conversion therapy].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2024
Same author

The glass ceiling of endometriosis surgeons is research.

Facts, views & vision in ObGyn·2024

Area of Science:

  • Genetics and Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Fluorescence in situ hybridization (FISH) is a key technique for mapping genetic loci on chromosomes.
  • Existing FISH methods face challenges in accurately ordering loci, especially those further apart due to chromatin looping.
  • Accurate locus order is crucial for gene mapping and understanding genomic structure.

Purpose of the Study:

  • To develop Bayesian statistical methods for improving locus order determination using FISH data.
  • To quantify the probability of correctly inferred locus orders from FISH experiments.
  • To propose efficient FISH experimental design strategies for gene map construction.

Main Methods:

  • Derivation of Bayesian methods to analyze two- and three-color FISH data from metaphase and interphase chromosomes.

Related Experiment Videos

  • Combination of results from multiple locus analyses to estimate multilocus order probability.
  • Application of methods to FISH mapping data of 14 markers in the BRCA1 region.
  • Proposal of bisection and trisection strategies for experimental design.

    • Main Results:

    • Developed Bayesian methods provide a probabilistic framework for evaluating locus order accuracy in FISH.
    • The methods successfully applied to BRCA1 region data, demonstrating utility in real-world mapping.
    • Proposed experimental strategies offer optimal performance for ordering loci, especially those < 1 Mb apart.
    • The approach addresses the inherent uncertainties in FISH-based locus ordering.

    Conclusions:

    • Bayesian analysis significantly enhances the accuracy and reliability of locus ordering using FISH.
    • The developed methods and strategies provide robust tools for precise gene map construction.
    • This work offers a statistically rigorous approach to interpreting FISH mapping data and improving genomic resolution.