Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mixed self-assembled monolayers in chemical separations

M J Wirth1, R W Fairbank, H O Fatunmbi

  • 1Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.

Science (New York, N.Y.)
|January 3, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Time-Resolved Fluorescence Intensity and Anisotropy Decays of 2,5-Diphenyloxazole by Two-Photon Excitation and Frequency-Domain Fluorometry.

The Journal of physical chemistry·2019
Same author

Surface-confined living radical polymerization for coatings in capillary electrophoresis.

Analytical chemistry·2011
Same author

Electrostatic Interactions between Ru(bpy)(3)(2+) and Chromatographic Surfaces.

Analytical chemistry·2011
Same author

Spectroscopic Probing of Mixed-Mode Adsorption of Ru(bpy)(3)(2+) to Silica.

Analytical chemistry·2011
Same author

Detector nonlinearity in frequency-domain fluorometry.

Applied optics·2010
Same author

Chemical analysis in small domains: introduction.

Chemical reviews·2001
Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026
Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026
Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026
Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026
Same journal

Retraction.

Science (New York, N.Y.)·2026
Same journal

Making time.

Science (New York, N.Y.)·2026
See all related articles

Researchers developed novel self-assembled monolayers to improve chemical separations. This method reduces electrostatic interactions, enhancing the separation of biomolecules like cytochrome c.

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biochemistry

Background:

  • Chromatographic separations of biomolecules and pharmaceuticals are often hindered by electrostatic interactions with separation media surfaces.
  • Silanol groups on silica surfaces can dissociate, leading to unwanted electrostatic effects.

Purpose of the Study:

  • To investigate the use of mixed self-assembled monolayers (SAMs) to reduce electrostatic interactions in chromatographic separations.
  • To improve the separation of charged biomolecules by modifying silica surfaces.

Main Methods:

  • Fabrication of mixed self-assembled monolayers (SAMs) using octadecyl and methyl chains on silica surfaces.
  • Utilizing molecular modeling to predict monolayer structure and cross-linking.
  • Employing silicon-29 nuclear magnetic resonance (NMR) spectroscopy to analyze monolayer composition and cross-linking.

Related Experiment Videos

  • Conducting chromatographic measurements to assess the reduction in electrostatic interactions.

    • Main Results:

    • Mixed SAMs form dense, two-dimensionally cross-linked networks, reducing silanol acid dissociation.
    • Molecular models and NMR confirmed steric possibility and predominance of cross-linking in mixed methylsiloxane monolayers.
    • Chromatographic data verified a significant reduction in electrostatic interactions with primarily methylsiloxane SAMs.

    Conclusions:

    • Self-assembled monolayers, particularly those rich in methylsiloxane, effectively minimize electrostatic interferences in chromatography.
    • This approach shows significant promise for the advanced separation of complex biomolecules, including charged proteins like cytochrome c.