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Related Experiment Videos

Poly(ethylene glycol) fluorescent linkers

A Pendri1, A Martinez, J Xia

  • 1Enzon, Inc., Piscataway, New Jersey 08854, USA.

Bioconjugate Chemistry
|September 1, 1995
PubMed
Summary
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Researchers synthesized novel PEG fluorescent linkers (PFL) using the dansyl group. These PFLs enable accurate PEGylation quantification in protein and drug conjugates, including taxol.

Area of Science:

  • Bioconjugation Chemistry
  • Fluorescent Probes
  • Polymer Science

Background:

  • Polyethylene glycol (PEG)ylation is crucial for improving drug pharmacokinetics and reducing immunogenicity.
  • Accurate quantification of PEGylation is essential for drug development and quality control.
  • Existing methods for PEGylation assessment can be complex or lack sensitivity.

Purpose of the Study:

  • To synthesize and characterize novel polyethylene glycol (PEG) fluorescent linkers (PFL) incorporating the dansyl group.
  • To establish a reliable method for quantifying PEGylation in protein and drug conjugates using PFLs.
  • To demonstrate the applicability of this method to both protein and small molecule drug conjugates.

Main Methods:

  • Synthesis of PEG linkers functionalized with the dansyl fluorophore.

Related Experiment Videos

  • Determination of quantum yields for the synthesized PEG fluorescent linkers.
  • Application of PFLs to quantify PEGylation in protein-drug conjugates and taxol conjugates.

    • Main Results:

    • Successful synthesis of the first PEG linkers containing the highly fluorescent dansyl group.
    • Quantification of quantum yields for the developed PEG fluorescent linkers.
    • Demonstrated utility of PFLs for calculating the PEG number in various protein conjugates.
    • Validated the method's applicability to lower molecular weight drugs, such as taxol.

    Conclusions:

    • Novel PEG fluorescent linkers (PFL) incorporating the dansyl group have been successfully synthesized.
    • The developed PFLs provide a sensitive and accurate method for quantifying PEGylation.
    • This method is versatile and applicable to a wide range of conjugates, including proteins and small molecule drugs.