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[Rheologic changes in stored erythrocyte concentrates]

E P Zilow1, G Zilow, P Ruef

  • 1Abteilung Neonatologie, Universität Heidelberg, Deutschland.

Beitrage Zur Infusionstherapie Und Transfusionsmedizin = Contributions to Infusion Therapy and Transfusion Medicine
|January 1, 1996
PubMed
Summary

PAGGS-M preservation solution maintained superior red blood cell rheological properties compared to SAG-M during storage. This suggests PAGGS-M may enhance oxygen transport and tissue perfusion.

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Area of Science:

  • Transfusion Medicine
  • Biotechnology
  • Hematology

Background:

  • Red blood cell (RBC) concentrates require preservation solutions to maintain viability and function during storage.
  • Assessing the impact of different preservation solutions on RBC rheology is crucial for optimizing blood product efficacy.
  • Current storage solutions like SAG-M have limitations in preserving RBC deformability and flow characteristics over extended periods.

Purpose of the Study:

  • To compare the effects of two preservation solutions, SAG-M (S-RBC) and PAGGS-M (P-RBC), on RBC rheological properties.
  • To evaluate changes in mean corpuscular volume (MCV), red cell deformability, and flow in narrow tubes over 42 days of storage.
  • To determine which preservation solution offers superior rheological characteristics for RBC concentrates.

Main Methods:

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  • Blood from 10 healthy donors was divided into two aliquots and preserved in either SAG-M or PAGGS-M.
  • Samples were analyzed at multiple time points (days 0, 7, 14, 28, 42) for MCV, whole cell deformability, and capillary viscosity.
  • MCV was measured using a Du Pont cell counter, deformability with a Myrenne Rheodyn, and flow properties via capillary viscosimetry.

Main Results:

  • P-RBC (PAGGS-M) maintained a constant MCV throughout storage, while S-RBC (SAG-M) showed an increase in MCV and a decrease in MCHC.
  • P-RBC exhibited stable deformability and viscosity reduction, whereas S-RBC demonstrated decreased deformability and less pronounced viscosity reduction over time.
  • PAGGS-M preserved superior rheological properties compared to SAG-M.

Conclusions:

  • PAGGS-M demonstrates superior preservation of RBC rheological properties, including MCV stability, deformability, and flow characteristics, compared to SAG-M.
  • The improved rheology of P-RBC suggests a potential for enhanced hemoglobin flux and oxygen transport to tissues.
  • PAGGS-M may represent a more effective preservation solution for RBC concentrates, potentially improving clinical outcomes.