Anti-arrestin immunoreactivity in the human retina: difference between light- and dark-adaptation

Differences in arrestin (Arr) immunolocalization between light(LA)- and dark(DA)-adapted retinae have been described in various species. We have for the first time studied 5 LA and 5 DA human retinae from surgically enucleated eyes, each group comprising 1 exenteration specimen and globes with malignant melanoma or 2 degrees glaucoma. To examine the distribution of Arr, immunohistochemistry was performed on paraffin sections using three different antibodies to Arr: a48k, 3D1.2, and 5c6.47. Immunoreactivity (IR) was tested using the avidin-biotin method, and results were visualized with diaminobenzidine. With both a48k and 5c6.47, labeling was most intense in the photoreceptor layer. When comparing LA and DA retinae, IR of photoreceptor outer segments (OS) was clearly different with very little IR in OS of DA but distinct positivity in OS of LA retina. This was most obvious in melanoma eyes where retinal morphology was well-preserved while in glaucomatous eyes with retinal degeneration this pattern was less apparent. 3D1.2 did not react in any of the specimens. Although there was some variation within each group, we demonstrated a distinct difference in anti-Arr IR between LA and DA specimens. Thus Arr-IR might now be used as a promising tool to further study retinal diseases on human surgical specimens involving photoreceptor degeneration.