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Molecular evidence for pap-G specific adhesion of Escherichia coli to human renal cells
M Söderhäll1, U S Bergerheim, S H Jacobson
1Department of Nephrology, Karolinska Hospital, Stockholm, Sweden.
Purpose:
To study the interaction between class II G-adhesin of Escherichia coli and human urogenital cells.
Material And Methods:
The adherence of two wild type P-fimbriated E. coli strains, both carrying a class II G-adhesion, and two constructed mutants (one class II G-adhesion knock-out mutant and one class switch mutant in which the papG gene was exchanged with a prsJ96 allele which is a representative of the class III G-adhesin) to human urogenital cells were examined by light microscopy and flow cytometry.
Results:
The wild type E. coli strains adhered avidly to proximal tubular cells, but the isogenic mutant strains did only adhere in one of the experiments. A soluble receptor analogue inhibited bacterial attachment.
Conclusions:
These experiments strongly suggest that the papG class II tip adhesin of P-fimbriae is essential in the pathogenesis of human kidney infection.