Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Urethral lymphocyte isolation in non-gonococcal urethritis

M Shahmanesh1, P G Pandit, R Round

  • 1Department of Genitourinary Medicine, Whittall Street Clinic, Birmingham, UK.

Genitourinary Medicine
|October 1, 1996
PubMed
Summary

This study found that urethral lymphocyte counts in non-gonococcal urethritis (NGU) differ based on infection type and recurrence. Lower lymphocyte levels in recurrent chlamydia-negative NGU suggest varied causes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical outcomes after first-line HIV treatment failure in South Africa: the next cascade of care.

HIV medicine·2020
Same author

Home-based intervention to test and start (HITS) protocol: a cluster-randomized controlled trial to reduce HIV-related mortality in men and HIV incidence in women through increased coverage of HIV treatment.

BMC public health·2019
Same author

Sexualized drug use ('chemsex') and high-risk sexual behaviours in HIV-positive men who have sex with men.

HIV medicine·2018
Same author

Perioperative passport: empowering people with diabetes along their surgical journey.

Diabetic medicine : a journal of the British Diabetic Association·2017
Same author

Porokeratosis Palmaris Et Plantaris- Punctata.

Indian journal of dermatology, venereology and leprology·2017
Same author

Erratum to "Brief interventions to prevent sexually transmitted infections suitable for in-service use: A systematic review" [Prev. Med. 91 (2016) 364-382].

Preventive medicine·2016

Area of Science:

  • Immunology
  • Urology
  • Microbiology

Background:

  • Non-gonococcal urethritis (NGU) is a common infection with varied causes.
  • Understanding the local immune response in NGU is crucial for diagnosis and treatment.
  • Previous studies have not fully elucidated the lymphocyte subsets involved in NGU pathogenesis.

Purpose of the Study:

  • To investigate the local immune response in patients with non-gonococcal urethritis (NGU).
  • To compare urethral lymphocyte populations between different types of NGU and gonorrhoea.
  • To assess if lymphocyte content differs between first and subsequent episodes of NGU.

Main Methods:

  • Urethral lymphocytes were isolated from first void urine (FVU) of NGU and gonorrhoea patients.
  • Magnetic beads targeting CD2 (pan-T cell marker) or CD4 were used for lymphocyte extraction.

Related Experiment Videos

  • CD2+ and CD4+ lymphocyte counts were compared between chlamydia-positive NGU, chlamydia-negative NGU, and gonorrhoea, as well as between first and recurrent NGU episodes.

    • Main Results:

    • Median CD2+ lymphocyte content was significantly higher in gonorrhoea and chlamydia-positive NGU compared to chlamydia-negative NGU.
    • Chlamydia-negative NGU showed significantly lower CD2+ lymphocyte counts (15.6 x 10^4) than chlamydia-positive NGU (35.2 x 10^4) and gonorrhoea (67.7 x 10^4).
    • First episodes of chlamydia-negative NGU had higher CD2+ cell counts (21.8 x 10^4) than recurrent episodes (12.3 x 10^4), but this was not observed in chlamydia-positive NGU. No differences in CD4+ cells were found.

    Conclusions:

    • The lower lymphocyte content in recurrent chlamydia-negative NGU suggests potentially different underlying causes compared to initial infections.
    • Sufficient lymphocytes can be isolated from urethral samples in NGU patients, enabling further studies on lymphocyte subsets.
    • These findings support the feasibility of using FVU for immunological investigations in NGU.