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RAG1 and RAG2 in developing rabbit appendix subpopulations

P Fuschiotti1, M G Fitts, R Pospisil

  • 1Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|January 1, 1997
PubMed
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Young rabbit appendix contains RAG gene products, indicating it

Area of Science:

  • Immunology
  • Developmental Biology
  • Molecular Biology

Background:

  • The rabbit appendix is a key site for B cell diversification and selection.
  • Previous studies on RAG2 protein in chicken bursa did not support its role in gene conversion.

Purpose of the Study:

  • To investigate the presence and role of RAG gene products in the young rabbit appendix.
  • To determine if RAG2 protein is present in rabbit appendix lymphocytes.

Main Methods:

  • Appendix cells from young rabbits (6-9 weeks old) were stained and sorted based on CD43 and IgM surface markers.
  • RAG1 transcripts and RAG2 protein were detected in specific cell populations.
  • Anti-rabbit RAG2 antibodies were produced to detect RAG2 protein.

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Main Results:

  • RAG1 transcripts and RAG2 protein were found in CD43+IgM- and CD43-IgM- appendix cells.
  • RAG2 protein was detected in all four subpopulations of rabbit appendix lymphocytes.
  • The presence of RAG gene products suggests the presence of pro-/pre-B cells.

Conclusions:

  • The young rabbit appendix serves as a site for both B cell development and diversification.
  • RAG gene products appear during various B cell maturation stages in the rabbit appendix.
  • These findings support the appendix's role in B cell maturation and gene diversification.