Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Methotrexate in gynecologic oncology]

S Isonishi1, Y Terashima

  • 1Dept. of Obstetrics & Gynecology, Jikei University School of Medicine.

Gan to Kagaku Ryoho. Cancer & Chemotherapy
|December 1, 1996
PubMed
Summary

Methotrexate, a chemotherapy agent, inhibits dihydrofolate reductase (DHFR), crucial for cancer cell growth. It effectively treats gestational trophoblastic disease and cervical cancer, with combination therapies improving outcomes for high-risk patients.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Soymilk-fermented with <i>Lactobacillus delbrueckii</i> subsp. <i>delbrueckii</i> TUA4408L improves immune-health in pigs.

Beneficial microbes·2022
Same author

Interleukin-6 and tumour necrosis factor-α cooperatively promote cell cycle regulators and proliferate rheumatoid arthritis fibroblast-like synovial cells.

Scandinavian journal of rheumatology·2019
Same author

Usefulness of ascites cytology for the evaluation of chemotherapy response in ovarian carcinosarcoma.

Cytopathology : official journal of the British Society for Clinical Cytology·2018
Same author

Selective interference of mTORC1/RAPTOR protects against human disc cellular apoptosis, senescence, and extracellular matrix catabolism with Akt and autophagy induction.

Osteoarthritis and cartilage·2017
Same author

Attenuation of pain behaviour by local administration of alpha-2 adrenoceptor antagonists to dorsal root ganglia in a rat radiculopathy model.

European journal of pain (London, England)·2015
Same author

Differing effects of liraglutide on gastric emptying in Japanese patients with type 2 diabetes.

Diabetes, obesity & metabolism·2013

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Context:

  • Methotrexate is a cornerstone chemotherapy agent.
  • It targets dihydrofolate reductase (DHFR), inhibiting DNA synthesis.
  • Resistance mechanisms include elevated DHFR activity or impaired drug transport.

Purpose:

  • To review the mechanism of action, resistance, and clinical applications of methotrexate.
  • To highlight its efficacy in gestational trophoblastic disease (GTD) and cervical cancer.
  • To discuss the role of combination chemotherapy in managing advanced disease.

Summary:

  • Methotrexate inhibits DHFR, blocking folic acid metabolism and DNA synthesis, leading to cancer cell death.
  • It achieves high cure rates in nonmetastatic GTD and significant remission in low-risk metastatic GTD.
  • Combination regimens like MAC and EMA-CO show 60-80% remission rates in high-risk metastatic GTD.

Impact:

  • Methotrexate has revolutionized GTD treatment, achieving near-universal cures for nonmetastatic cases.
  • It offers a viable treatment option for metastatic squamous cell carcinoma of the cervix.
  • Understanding resistance mechanisms and optimizing combination therapies are key to improving patient survival and outcomes.

Related Experiment Videos