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A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences
B W Blais1, G Turner, R Sooknanan
1Laboratory Services Division, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada.
Applied and Environmental Microbiology
|January 1, 1997
Abstract:
A nucleic acid sequence-based amplification system primarily targeting mRNA from the Listeria monocytogenes hlyA gene was developed. This system enabled the detection of low numbers (< 10 CFU/g) of L. monocytogenes cells inoculated into a variety of dairy and egg products after 48 h of enrichment in modified listeria enrichment broth.