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Updated: Jul 11, 2026

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Published on: August 6, 2011
Development of a species-specific gene probe for Hyphomicrobium facilis with the inverse PCR
A Fesefeldt1, M Poetsch, C G Gliesche
1Institut für Allgemeine Mikrobiologie, Universität Kiel, Germany.
Abstract:
A species-specific gene probe for Hyphomicrobium facilis was generated from a transposon Tn5-132 insertion mutant defective in methanol oxidation by the inverse PCR. With this probe, the abundance of H. facilis in a garden soil was determined as a subfraction of the total cultivable hyphomicrobia.
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