Phylogenetic relationships in A-genome species of rice as revealed by RAPD analysis

In order to clarify the phylogenetic relationships in A-genome species of rice, RAPD analysis was carried out using 29 accessions (8 Oryza sativa, 6 O. glaberrima, 13 O. perennis, and 2 O. breviligulata). Their total DNA was used as template for PCR with 14 decamer primers. The amplified products were electrophoresed in agarose gel, and the banding patterns were observed. Average number of total amplified bands for each accession using 14 primers was 84.6. By comparing the banding patterns, the proportion of common bands between 29 accessions was calculated and a dendrogram showing genetic relationships was constructed. Most of the accessions formed clusters corresponding to their taxonomic grouping. The Oceanian and African forms of O. perennis were substantially different from all other groups. The Asian form of O. perennis, however, formed a complex with O. sativa while O. breviligulata was clustered with O. glaberrima. This indicates that O. sativa and O. glaberrima have probably originated from the Asian form of O. perennis and from O. breviligulata, respectively.