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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
Published on: September 20, 2018
Benzoyl peroxide modulates gene expression by epigenetic mechanism in mouse epidermal JB6 cells
1Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.
Abstract:
The mechanism by which an oxidant tumor promoter benzoyl peroxide (BP) influences signal transduction, was studied in promotion insensitive (P-), promotion sensitive (P+) and transformed (Tx) mouse epidermal JB6 cells. The basal levels of antioxidant enzymes CuZn-superoxide dismutase and catalase were higher in P+ and Tx JB6 cells. BP decreased the activities of these enzymes transiently in P+ and Tx cells, but induced them in P- JB6 cells. BP increased poly ADPR polymerase activity accompanied by a drop in NAD+ levels more significantly in P- JB6 cells, but did not affect PKC activity. It induced c-jun and c-fos gene expression in JB6 variants but to different extents, suggesting that it mediates its effects via genetic- epigenetic mechanism(s).
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