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Identification and characterization of receptor for dengue virus-induced macrophage cytotoxin
P D Khare1, R Dhawan, U C Chaturvedi
1Postgraduate Department of Microbiology, K.G.Medical College, Lucknow, India.
Abstract:
The present study was undertaken to identify the receptor for dengue virus type 2 (DV) induced macrophage cytotoxin (CF2) on mouse peritoneal macrophages (MPhi). The binding of 125I-labelled CF2 to MPhi was saturable (15 nM), reversible, temperature, pH- and time-dependent. The saturation concentration was similar to that causing cell death. Scatchard analysis showed the presence of intermediate type of affinity receptor and the number of receptor sites was 1.1 x 10(6) per cell with dissociation constant of 14.28 nM.
Insights
Researchers identified the receptor for dengue virus type 2 (DV) cytotoxin (CF2) on mouse macrophages. Binding studies revealed specific receptor characteristics, including affinity and site number, crucial for understanding DV
Area of Science:
- Immunology
- Virology
- Cell Biology
Background:
- Dengue virus type 2 (DV) infection can induce macrophage cytotoxin (CF2) production.
- The specific cellular receptor for CF2 on macrophages remains unidentified.
Purpose of the Study:
- To identify and characterize the receptor for DV-induced macrophage cytotoxin (CF2) on mouse peritoneal macrophages (MPhi).
Main Methods:
- Radioligand binding assays using 125I-labelled CF2.
- Scatchard analysis to determine receptor affinity and number.
Main Results:
- 125I-CF2 binding to MPhi was saturable, reversible, and dependent on temperature, pH, and time.
- Scatchard analysis indicated an intermediate affinity receptor with 1.1 x 10^6 sites/cell and a Kd of 14.28 nM.
Conclusions:
- A specific receptor for DV-induced macrophage cytotoxin (CF2) exists on mouse peritoneal macrophages.
- This receptor exhibits intermediate affinity and its binding characteristics correlate with cytotoxic effects.