Related Experiment Videos
Cytokine induction of platelet activation
J A Lumadue1, S M Lanzkron, S D Kennedy
1Department of Pathology and Medicine, Johns Hopkins Hospital, Baltimore, Maryland 21287-7061, USA.
American Journal of Clinical Pathology
|December 1, 1996
Summary
Cytokines accumulating in stored platelets can activate them, impacting transfusion viability. Reducing cytokine generation may improve platelet function and safety.
Area of Science:
- Hematology
- Transfusion Medicine
- Immunology
Background:
- Accumulation of cytokines in stored platelet concentrates is linked to transfusion reactions.
- Further investigation is needed to understand additional adverse effects of cytokine accumulation.
Purpose of the Study:
- To determine if cytokines that accumulate during routine platelet storage can induce platelet activation in vitro.
- To assess the impact of cytokine levels on platelet function.
Main Methods:
- Quantified concentrations of Interleukin-6 (IL-6) and Interleukin-8 (IL-8) in random donor platelet concentrates over 4 days of storage.
- Incubated fresh platelets with exogenous cytokine concentrations mirroring those found in stored platelets.
- Measured platelet activation using flow cytometry and a p-Selectin antibody.
Main Results:
- Significant platelet activation was observed when platelets were incubated with cytokine concentrations typical of days 3 and 4 of storage.
- Demonstrated that routinely accumulating cytokine levels in stored platelets can alter platelet biology.
Conclusions:
- Cytokine accumulation during platelet storage can lead to platelet activation.
- Strategies aimed at reducing cytokine generation may enhance the function and viability of stored platelets for transfusion.