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Antimutagens from Plumeria acuminata Ait
A P Guevara1, E Amor, G Russell
1Institute of Chemistry, College of Science, University of the Phillippines (UP), Diliman, Quezon City, Philippines.
Mutation Research
|December 12, 1996
Summary
Green leaves of Plumeria acuminata contain potent antimutagens. Four compounds, including ursolic acid and lupeol carboxylic acid, significantly reduced mutagenicity in mice, highlighting their potential as natural chemoprotective agents.
Area of Science:
- Phytochemistry
- Pharmacology
- Toxicology
Background:
- Plumeria acuminata Ait is a plant with traditional medicinal uses.
- Natural products are a rich source of bioactive compounds with potential health benefits.
- Mutagenicity poses a significant health risk, necessitating the search for effective antimutagenic agents.
Purpose of the Study:
- To isolate and identify antimutagenic compounds from Plumeria acuminata Ait.
- To evaluate the efficacy of isolated compounds in preventing chemically induced mutagenicity in vivo.
- To explore the potential of Plumeria acuminata Ait as a source of natural antimutagens.
Main Methods:
- Bioactivity-directed fractionation of Plumeria acuminata Ait leaf extract using hexane and carbon tetrachloride fractions.
- Isolation of compounds using chromatographic techniques.
- Assessment of antimutagenic activity using the in vivo micronucleus test in mice challenged with mitomycin C.
Main Results:
- Four isolates (A1, C1, D3, F2) exhibited significant antimutagenic activity.
- Identified compounds include stigmast-7-enol (C1), lupeol carboxylic acid (D3), and ursolic acid (F2).
- Compound A1, with a suggested long hydrocarbon chain, showed 75% reduction in micronucleated polychromatic erythrocytes (MPCE), while C1, D3, and F2 reduced MPCE by 80%, 57%, and 76%, respectively.
Conclusions:
- Plumeria acuminata Ait leaf extract contains potent antimutagenic compounds.
- Ursolic acid, lupeol carboxylic acid, and stigmast-7-enol demonstrate significant antimutagenic properties.
- These findings support the investigation of Plumeria acuminata Ait for developing natural chemoprotective agents.