A sialic acid-binding lectin from ovine placenta: purification, specificity and interaction with actin

M M Iglesias¹, G D Cymes, C Wolfenstein-Todel

¹Instituto de Química y Fisicoquimica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.

|December 1, 1996

Abstract:

A sialic-acid-specific lectin from ovine placental cotyledons was purified by affinity chromatography on bovine submaxillary mucin-agarose followed by gel filtration, and it showed a molecular weight of 65000 by sodium dodecylsulfate-polyacrylamide gel electrophoresis. This lectin has the capacity to interact with actin, since it binds to actin-F in a cosedimentation assay and it acts as a mediator in the binding of actin to the affinity column. The lectin agglutinated rabbit and rat erythrocytes, but not human A, B or O erythrocytes. Haemagglutination inhibition assays of different saccharides, glycoproteins and glycolipids indicate that this lectin has affinity for sialic acid, which is enhanced by its O-acetylation. The N-terminal sequence of the protein shows 92% identity with rabbit and porcine uterine calreticulin.

Related Experiment Videos

A sialic acid-binding lectin from ovine placenta: purification, specificity and interaction with actin

Related Concept Videos

Isolation of galectin-1 from human platelets: its interaction with actin.

Galectins: matricellular glycan-binding proteins linking cell adhesion, migration, and survival.

Identification of gp17 glycoprotein and characterization of prostatic acid phosphatase (PAP) and carboxypeptidase E (CPE) fragments in a human seminal plasma fraction interacting with concanavalin A.

Regulated expression of galectin-1 during B-cell activation and implications for T-cell apoptosis.

Specificity of the binding site of the sialic acid-binding lectin from ovine placenta, deduced from interactions with synthetic analogues.

Ovine placental lactogen and ovine prolactin: partial proteolysis and conformational stability.

Structural dynamics and stability analysis of a novel ARSK variant confirms its role in MPS type X pathogenesis.

Homo-oligomeric forms of lectins: their structural insights for glycan binding from the genus Canavalia.

Mechanistic and functional integration of sirtuin deacylases: from substrate specificity to biological outcomes.

Macrophage modulation accounts for the anti-inflammatory effect of Hypnea cervicornis agglutinin in rat arthritis induced by zymosan.

Nucleolar-localized sialidase Neu4 suppresses cell proliferation through destabilizing c-Myc via interacting with ribosomal proteins L5 and L11.

3'-sialyllactose inhibits the migration and invasion of LPS-induced B16F10 mouse melanoma cells through inactivation of the NF-KB signaling pathway.

Halogenated Tryptophans Improve Integrin αVβ6 Affinity of Cyclic RGD Peptides and Provide Handles for Late-Stage Derivatization.

Affinity Peptide-Driven Precision Glycan Analysis: From Targeted Capture to Single-Molecule Profiling.

Urinary Glucose Concentration as a Predictor of Proteinuria Reduction in Chronic Kidney Disease Patients Treated with Sodium-Glucose Cotransporter 2 Inhibitors.

Effects of Astragaloside IV Combined With Metronidazole on Cyst Shedding and Mucosal Immune and Inflammatory Responses in a Murine Model of Giardiasis.

Maximizing weight loss with cagrisema: a systematic review and GRADE-assessed meta-analysis of randomized controlled trials.

Heparin Modulates SARS-CoV-2 Spike-Platelet Factor 4 Interactions through Concentration-Dependent Interface Remodeling.

Related Experiment Videos

A sialic acid-binding lectin from ovine placenta: purification, specificity and interaction with actin

Abstract:

Related Concept Videos

Related Articles