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Related Experiment Videos

Undocumented embryos: do not trash them, FISH them

D Manor1, S Kol, N Lewit

  • 1Department of Obstetrics and Gynecology, Rambam Medical Center and Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.

Human Reproduction (Oxford, England)
|November 1, 1996
PubMed
Summary
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Undocumented embryos from in-vitro fertilization (IVF) may be viable. Fluorescent in-situ hybridization (FISH) analysis confirmed diploidy in 57% of these embryos, with one resulting in a successful pregnancy.

Area of Science:

  • Reproductive biology
  • Genetics
  • Assisted reproductive technology

Background:

  • Pronuclei assessment is standard for evaluating embryo viability post-in-vitro fertilization (IVF).
  • Occasionally, pronuclei disappear before assessment, leading to 'undocumented' embryos that are typically discarded due to uncertain ploidy status.
  • Fluorescent in-situ hybridization (FISH) offers a method to assess chromosomal status in early-cleaving embryos.

Purpose of the Study:

  • To investigate the chromosomal status of undocumented embryos using FISH.
  • To determine if undocumented embryos can lead to successful pregnancies.
  • To evaluate the utility of FISH in salvaging undocumented embryos for potential transfer.

Main Methods:

  • FISH analysis was performed on biopsied blastomeres from 23 undocumented embryos from 10 patients.

Related Experiment Videos

  • Probes targeted five chromosomes: X, Y, 13, 18, and 21.
  • Embryos were assessed for diploidy and chromosomal anomalies.

    • Main Results:

    • Diploidy was confirmed in 13 (57%) of the 23 analysed embryos.
    • Ten embryos exhibited chromosomal abnormalities.
    • Six diploid embryos were transferred, resulting in one ongoing pregnancy from an embryo that had already cleaved by 20 hours post-insemination.

    Conclusions:

    • Accelerated pronuclear membrane breakdown and cleavage do not necessarily indicate aneuploidy.
    • FISH analysis can identify viable undocumented embryos, increasing embryo availability for transfer.
    • This approach may improve IVF success rates, particularly in patients with limited embryo numbers.