Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An improved animal model for studying desferrioxamine

A Steward1, I Williamson, T Madigan

  • 1Department of Drug Discovery, Ciba Pharmaceuticals, Horsham, West Sussex.

British Journal of Haematology
|December 1, 1996
PubMed
Summary

A new hamster model effectively studies desferrioxamine, revealing subcutaneous administration is most effective for iron removal. This model aids in optimizing desferrioxamine use for iron chelation therapy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of a pilot sensory play intervention to increase fruit acceptance in preschool children.

Appetite·2017
Same author

Exploring lesbian, gay and bisexual patients' accounts of their experiences of cancer care in the UK.

European journal of cancer care·2016
Same author

Reduction En Masse of a Strangulated Inguinal Hernia.

Canadian Medical Association journal·2010
Same author

A double-blind randomised placebo-controlled trial of topical intranasal corticosteroids in 4- to 11-year-old children with persistent bilateral otitis media with effusion in primary care.

Health technology assessment (Winchester, England)·2009
Same author

Acute sinusitis: which factors do FPs believe are most diagnostic and best predict antibiotic efficacy?

The Journal of family practice·2006
Same author

Grommets (ventilation tubes) for hearing loss associated with otitis media with effusion in children.

The Cochrane database of systematic reviews·2005

Area of Science:

  • Pharmacology
  • Animal Models
  • Iron Metabolism

Background:

  • Desferrioxamine is a key chelator for iron overload disorders.
  • Understanding optimal administration routes is crucial for therapeutic efficacy.
  • Existing models may not fully replicate human desferrioxamine pharmacokinetics.

Purpose of the Study:

  • To establish and validate a hamster model for desferrioxamine research.
  • To compare the efficacy of different desferrioxamine administration routes in iron excretion.
  • To assess the sensitivity of the model in detecting iron elimination differences.

Main Methods:

  • Development of a desferrioxamine hamster model.
  • Intravenous injection of [59Fe]ferritin into hamsters.
  • Administration of desferrioxamine via subcutaneous, intravenous, and oral routes.

Related Experiment Videos

  • Quantification of iron excretion, primarily into the intestines.

    • Main Results:

    • The hamster model mimics human plasma stability and metabolite formation of desferrioxamine.
    • Subcutaneous desferrioxamine administration showed the highest iron excretion (10.5%).
    • Intravenous (6.25%) and oral (3.66%) routes were less effective than subcutaneous.
    • A dose-response relationship was observed with intravenous administration.

    Conclusions:

    • The developed hamster model is a sensitive tool for studying desferrioxamine.
    • Subcutaneous administration is the most effective route for desferrioxamine-mediated iron removal in this model.
    • This model facilitates the comparison of administration routes to optimize desferrioxamine therapy.