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Related Experiment Videos

The mammalian brain rsec6/8 complex

S C Hsu1, A E Ting, C D Hazuka

  • 1Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University Medical School, California 94305-5428, USA.

Neuron
|December 1, 1996
PubMed
Summary
This summary is machine-generated.

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Researchers identified the mammalian rsec6/8 complex, crucial for neurotransmitter release. This protein complex interacts with syntaxin, aiding vesicle docking and fusion in nerve terminals.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • The yeast Sec6/8/15 complex is vital for exocytosis.
  • Mammalian brain possesses homologous components, rsec6 and rsec8, forming a 17S complex.

Purpose of the Study:

  • To characterize the mammalian rsec6/8 complex.
  • To investigate its role in neurotransmission.

Main Methods:

  • Purification and partial amino acid sequencing of the mammalian rsec6/8 complex.
  • Immunoprecipitation assays to study protein interactions.
  • Cellular localization studies in nerve terminals.

Main Results:

  • The mammalian rsec6/8 complex consists of eight novel proteins, totaling 743 kDa.

Related Experiment Videos

  • The complex is widely expressed in the brain and localizes to the plasma membrane of nerve terminals.
  • Membrane-associated rsec6/8 complex co-immunoprecipitates with syntaxin.

    • Conclusions:

    • The mammalian rsec6/8 complex is implicated in neurotransmitter release.
    • It likely interacts with the core vesicle docking and fusion machinery, including syntaxin.