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Related Experiment Videos

Abnormal desmoglein expression by squamous cell carcinoma cells

H Harada1, K Iwatsuki, M Ohtsuka

  • 1Department of Dermatology, Fukushima Medical College, Japan.

Acta Dermato-Venereologica
|November 1, 1996
PubMed
Summary
This summary is machine-generated.

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Squamous cell carcinoma cells show altered expression of desmoglein proteins, differing from normal keratinocytes. These desmoglein changes may influence tumor cell behavior, including invasion and metastasis.

Area of Science:

  • Cell Biology
  • Oncology
  • Dermatology

Background:

  • Cell adhesion molecules are crucial for tissue integrity.
  • Aberrant expression of these molecules is linked to various cancers, including squamous cell carcinoma.
  • Desmosomal cadherins, like desmogleins, play a key role in epithelial cell adhesion.

Purpose of the Study:

  • To compare the expression patterns of desmosomal cadherins and related proteins in human squamous cell carcinoma (SCC) cell lines and in vivo SCC with normal human keratinocytes.
  • To investigate potential alterations in desmoglein isoforms and their antigenic properties in SCC.
  • To explore the relationship between abnormal desmoglein expression and tumor cell kinetics.

Main Methods:

  • Comparison of protein expression using Western blotting.

Related Experiment Videos

  • Analysis of messenger RNA (mRNA) expression of desmogleins.
  • Immunological assessment using pemphigus vulgaris serum to detect antigenic epitopes.

    • Main Results:

    • Squamous cell carcinoma cells (in culture and in vivo) displayed diminished or unusual expression of desmoglein 3 and desmoglein 1 compared to normal keratinocytes.
    • Abnormal expression of E-cadherin, desmoplakin, and plakoglobin was also observed in SCC cells.
    • Western blotting revealed variations in desmoglein molecular weights and reactivity with pemphigus vulgaris serum in SCC lines.
    • SCC cell lines consistently expressed desmoglein 2 and desmoglein 3 mRNA, while normal keratinocytes expressed desmoglein 1 and desmoglein 3 mRNA.

    Conclusions:

    • Squamous cell carcinoma cells exhibit abnormal expression of desmoglein isoforms.
    • These molecular changes in desmogleins may correlate with tumor cell characteristics, potentially affecting invasion and metastasis.
    • Further research into desmoglein dysregulation in SCC could offer insights into cancer progression and therapeutic targets.