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Cutaneous microdialysis. Methodology and validation

L Groth1

  • 1Leo Pharmaceutical Products, Department of Dermatological Research, Ballerup, Denmark.

Acta Dermato-Venereologica. Supplementum
|January 1, 1996
PubMed
Summary
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Cutaneous microdialysis effectively studies hydrophilic substances in skin. However, this technique requires further development for accurate measurement of lipophilic compounds due to low recovery and trauma effects.

Area of Science:

  • Dermatological research
  • Pharmacokinetics
  • Biomedical engineering

Background:

  • Cutaneous microdialysis is a technique to measure substances in skin's extracellular fluid.
  • It involves inserting a probe into the dermis to collect dialysate for analysis.
  • This method is valuable for studying topical drug penetration in vivo.

Purpose of the Study:

  • To describe and validate the methodology of cutaneous microdialysis.
  • To assess its efficacy in studying the skin penetration of various topically applied substances.
  • To identify limitations and areas for improvement in the technique.

Main Methods:

  • Investigated glucose, sodium fusidate, betamethasone dipropionate, and calcipotriol using in vitro microdialysis.
  • Evaluated the impact of perfusion rate, membrane length, stirring, and temperature on substance recovery.

Related Experiment Videos

  • Assessed in vivo skin trauma responses (blood flow, erythema, thickness) and histamine release in rat and human skin.

    • Main Results:

    • In vitro recovery was influenced by substance properties; lipophilic compounds showed low and variable recovery.
    • In vivo probe insertion caused skin trauma, necessitating equilibration periods of 30-90 minutes.
    • Measurable concentrations of lipophilic drugs (betamethasone 17-valerate, fusidic acid) and calcipotriol were difficult to achieve via topical application.

    Conclusions:

    • Cutaneous microdialysis is most suitable for hydrophilic substances with low protein binding and molecular weight.
    • Further methodological development is needed for reliable in vivo analysis of lipophilic compounds.
    • The technique's utility is limited by variable recovery and probe-induced skin trauma.