Elastase-induced hydrolysis of synthetic solid substrates: poly(ester-urea-urethane) and poly(ether-urea-urethane)

¹Cardiovascular Devices Division, University of Ottawa Heart Institute, ON, Canada.

|December 1, 1996

Summary

Porcine pancreatic elastase (PPE) degraded poly(ester-urea-urethane) biomaterials more than human neutrophil elastase (HNE). This suggests implanted device stability may be impacted by inflammatory enzymes.

Area of Science:

Biomaterials Science
Enzymology
Polymer Chemistry

Background:

Implanted medical devices can elicit inflammatory responses.
Neutrophils are key inflammatory cells containing elastolytic enzymes.
The stability of polyurethane biomaterials in vivo is not fully understood.

Purpose of the Study:

To investigate the degradation of model polyurethanes by human neutrophil elastase (HNE) and porcine pancreatic elastase (PPE).
To compare the enzymatic degradation rates of poly(ester-urea-urethane) and poly(ether-urea-urethane) biomaterials.
To assess the potential impact of elastase activity on biomaterial stability during inflammation.

Main Methods:

Radiolabeling of model polyurethanes with [14C]toluene diisocyanate ([14C]TDI).
Incubation of radiolabeled poly(ester-urea-urethane) ([14C]TDI/PCL/ED) and poly(ether-urea-urethane) ([14C]TDI/PTMO/ED) with HNE and PPE.
Quantification of released radioactive carbon as a measure of degradation.
Inhibition studies using a specific elastase inhibitor.

Main Results:

Porcine pancreatic elastase (PPE) released ten-fold more radioactive carbon from [14C]TDI/PCL/ED than human neutrophil elastase (HNE).
A specific elastase inhibitor significantly reduced PPE-induced degradation.
[14C]TDI/PTMO/ED showed ten-fold less degradation by PPE compared to [14C]TDI/PCL/ED.

Conclusions:

Poly(ester-urea-urethane) biomaterials are more susceptible to degradation by PPE than poly(ether-urea-urethane) counterparts.
Elastase activity, particularly from neutrophils during inflammation, may compromise the stability of implanted polyurethane devices.
Further research is needed to understand the long-term effects of enzymatic degradation on biomaterial performance in vivo.

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Area of Science:

Background:

Purpose of the Study:

Main Methods:

Main Results:

Conclusions:

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