Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Upgrading unsatisfactory cervical smears with the MiB-1 method

M E Boon¹, E D Kleinschmidt-Guy, A Wijsman-Grootendorst

¹Leiden Cytology and Pathology Laboratory, The Netherlands.

Diagnostic Cytopathology

|November 1, 1996

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Guidelines for the Cytopathologic Diagnosis of Epithelioid and Mixed-Type Malignant Mesothelioma: a secondary publication.

Cytopathology : official journal of the British Society for Clinical Cytology·2015

Same author

Endometrial cells in liquid-based cervical cytology: a diagnostic pitfall solved by preparing cytohistology from the residual thin layer sample.

Acta cytologica·2011

Same author

Increased diagnostic accuracy of atypical glandular cells in cervical liquid-based cytology using cell blocks.

Cytopathology : official journal of the British Society for Clinical Cytology·2010

Same author

Theory and practice of combining coagulant fixation and microwave histoprocessing.

Biotechnic & histochemistry : official publication of the Biological Stain Commission·2008

Same author

Human papillomavirus DNA testing for the detection of cervical intraepithelial neoplasia grade 3 and cancer: 5-year follow-up of a randomised controlled implementation trial.

Lancet (London, England)·2007

Same author

Symptomatic candidiasis: Using self sampled vaginal smears to establish the presence of Candida, lactobacilli, and Gardnerella vaginalis.

Diagnostic cytopathology·2007

Restaining unsatisfactory cervical smears with MiB-1 enhances detection of abnormal cells. This method improves the diagnosis of precancerous and cancerous cervical conditions, especially in difficult-to-evaluate samples.

Area of Science:

Gynecologic Pathology
Cervical Cytology
Immunohistochemistry

Background:

Unsatisfactory cervical smears pose diagnostic challenges due to inflammation or blood obscuring cellular details.
Accurate diagnosis of cervical abnormalities is crucial for timely intervention and patient management.

Purpose of the Study:

To evaluate the utility of MiB-1 immunohistochemistry in restaining unsatisfactory cervical smears.
To assess the diagnostic yield and impact of MiB-1 on classifying cervical lesions.

Main Methods:

Restaining of 2,068 unsatisfactory cervical smears using MiB-1 antibody over 18 months.
Comparison of diagnostic findings between satisfactory and unsatisfactory smear groups.
Biopsy correlation for cases with significant MiB-1 findings.

Related Experiment Videos

Main Results:

MiB-1 restaining identified more abnormal smears in the unsatisfactory group compared to the satisfactory group.
Biopsies from the unsatisfactory group revealed severe dysplasia, carcinoma in situ, and invasive carcinoma.
The detection rate for invasive cervical carcinoma was ten times higher in the unsatisfactory group.

Conclusions:

MiB-1 immunohistochemistry is a valuable tool for improving the diagnostic accuracy of unsatisfactory cervical smears.
This method enhances the detection of significant cervical pathologies, including invasive cancer.
MiB-1 restaining offers improved diagnostic acumen for challenging cervical cytology samples.