Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tyrosine kinase signalling pathways

T Pawson1

  • 1Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto, Ontario, Canada.

Princess Takamatsu Symposia
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SYK interaction with ITGβ4 suppressed by Epstein-Barr virus LMP2A modulates migration and invasion of nasopharyngeal carcinoma cells.

Oncogene·2014
Same author

The ShcA adaptor activates AKT signaling to potentiate breast tumor angiogenesis by stimulating VEGF mRNA translation in a 4E-BP-dependent manner.

Oncogene·2014
Same author

The ShcA SH2 domain engages a 14-3-3/PI3'K signaling complex and promotes breast cancer cell survival.

Oncogene·2012
Same author

Generating a panel of highly specific antibodies to 20 human SH2 domains by phage display.

Protein engineering, design & selection : PEDS·2010
Same author

EphB2 and EphA4 receptors regulate formation of the principal inter-hemispheric tracts of the mammalian forebrain.

Neuroscience·2009
Same author

Effects of dasatinib on EphA2 receptor tyrosine kinase activity and downstream signalling in pancreatic cancer.

British journal of cancer·2008
Same journal

Survival after liver transplantation in patients with hepatocellular carcinoma.

Princess Takamatsu symposia·1995
Same journal

Surgical treatment of hepatocellular carcinoma.

Princess Takamatsu symposia·1995
Same journal

Treatment of unresectable hepatocellular carcinoma: targeted therapies using iodized oil.

Princess Takamatsu symposia·1995
Same journal

The present status of treatments for hepatocellular carcinoma.

Princess Takamatsu symposia·1995
Same journal

Prospects for prophylactic and therapeutic hepatitis C virus vaccines.

Princess Takamatsu symposia·1995
Same journal

Association of cytotoxic T lymphocyte (CTL) escape mutations with persistent hepatitis C virus (HCV) infection.

Princess Takamatsu symposia·1995
See all related articles

Protein-tyrosine kinases regulate cell growth by interacting with SH2 domains, which bind to tyrosine phosphorylation sites. These interactions activate signaling pathways, with adaptor proteins like Sem-5/drk/Grb2 linking kinases to downstream targets such as the Ras pathway.

Area of Science:

  • Cellular Biology
  • Molecular Signaling
  • Oncogenesis

Background:

  • Protein-tyrosine kinases are crucial for cell signaling, regulating growth and differentiation.
  • Aberrant kinase activity is implicated in oncogenesis, with constitutively active variants found in oncogenes.
  • Intracellular targets of tyrosine kinases often contain Src homology 2 (SH2) domains.

Purpose of the Study:

  • To elucidate the role of SH2 domains in mediating signals from tyrosine kinases.
  • To understand how SH2 and SH3 domains function as adaptors in signal transduction.
  • To investigate the specific mechanism of Ras pathway regulation by SH2/SH3-containing adaptor proteins.

Main Methods:

  • Analysis of protein-protein interactions mediated by SH2 and SH3 domains.

Related Experiment Videos

  • Identification of tyrosine phosphorylation sites recognized by SH2 domains.
  • Investigating the function of adaptor proteins in linking tyrosine kinases to downstream effectors.

    • Main Results:

    • SH2 domains specifically recognize and bind to tyrosine phosphorylation sites on activated receptors.
    • SH2-containing proteins, often with SH3 domains, act as adaptors, coupling kinases to downstream targets.
    • The adaptor protein Sem-5/drk/Grb2 exemplifies synergistic SH2/SH3 action, linking tyrosine kinases to the Ras pathway via Sos.

    Conclusions:

    • SH2 domains are key mediators of external signals received by protein-tyrosine kinases.
    • The interplay of SH2 and SH3 domains is critical for assembling signaling complexes and regulating cellular processes.
    • Adaptor proteins play a vital role in signal transduction, exemplified by the regulation of the Ras pathway.