[Modification of Bacillus intermedius ribonuclease by dialdehyde dextran]

Area of Science:

• Biochemistry
• Enzyme Engineering
• Protein Modification

Background:

• Bacillus intermedius ribonuclease is a valuable enzyme.
• Enzyme modification can improve stability and activity.
• Dialdehyde dextran is a potential carrier for enzyme immobilization.

Purpose of the Study:

• To optimize the modification conditions of Bacillus intermedius ribonuclease using dialdehyde dextran.
• To enhance the enzyme's activity and stability.
• To investigate the relationship between enzyme-carrier bonds and preparation characteristics.

Main Methods:

• Optimization of modification conditions using the Box and Wilson method.
• Evaluation of generalized parameters including enzyme protein yield and stability.
• Analysis of enzyme activity and preparation characteristics based on enzyme-carrier bonds.

Main Results:

• Optimized conditions led to Bacillus intermedius ribonuclease with higher activity.
• Enzyme stability during modification was improved.
• Yield of activity and preparation characteristics were found to depend on the number of enzyme-carrier bonds.

Conclusions:

• The Box and Wilson method effectively optimized the modification of Bacillus intermedius ribonuclease with dialdehyde dextran.
• Enhanced enzyme activity and stability were achieved.
• Controlling enzyme-carrier bonds is crucial for optimizing modified enzyme characteristics.