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Updated: Aug 6, 2026

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
Published on: December 27, 2016
An 15N NMR study of the protonation of two di-(alpha-pyridyl) compounds in solutions and the solid state
J Klimkiewicz1, L Stefaniak, E Grech
1Institute of Organic Chemistry, Polish Academy of Sciences, Warsaw, Poland.
Abstract:
15N NMR data are reported for two Di-(alpha-pyridyl) derivatives in various stages of protonation. A combination of 15N NMR measurements taken, on a number of solutions, and on the solid state provide a quantitative overall view of the progress of protonation of these compounds. 15N shieldings, J(15N-1H) and 2J (15N-1H) interactions all provide information indicative of unsymmetrical monoprotonation in the solid state and producing both the monoprotonated [N-H-N]+ structure and the diprotonated structure in the presence of an excess of TFA.
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