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Related Experiment Videos

Electric fields and proliferation in a chronic wound model

R Goldman1, S Pollack

  • 1Department of Rehabilitation Medicine, University of Pennsylvania, Philadelphia 19104, USA.

Bioelectromagnetics
|January 1, 1996
PubMed
Summary

Low-frequency electric fields, specifically between 37-50 mV/m at 10 Hz, significantly enhance human dermal fibroblast proliferation in a wound model. This finding aligns with endogenous field patterns in healing wounds.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Wound Healing Research

Background:

  • Decubitus and leg ulcers present significant clinical challenges.
  • Understanding endogenous electric fields in wound healing is crucial for developing novel therapies.
  • Existing wound models require further refinement to mimic in vivo conditions.

Purpose of the Study:

  • To investigate the effect of specific low-frequency electric fields on human dermal fibroblast proliferation in a collagen-based wound model.
  • To determine the optimal electric field parameters (amplitude and frequency) for stimulating cell growth.
  • To correlate in vitro findings with postulated endogenous electric field ranges in healing wounds.

Main Methods:

  • A human dermal fibroblast-populated type I collagen dermal equivalent matrix (DEM) was used as an in vitro wound model.

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  • DEM samples were exposed to sinusoidal electric currents at 10 and 100 Hz with varying amplitudes (18-1,000 mV/m).
  • Cell proliferation was assessed by measuring total DNA content and 3H-thymidine incorporation after electric field exposure.

    • Main Results:

    • A specific electric field amplitude window (37-50 mV/m) at 10 Hz significantly increased fibroblast proliferation.
    • At 41 mV/m and 10 Hz, total DNA increased by 70% (P < .01), and 3H-thymidine incorporation showed significant increases (P < .05).
    • The proliferative response was dependent on both electric field parameters and cell density within the matrix.

    Conclusions:

    • Low-frequency electric fields within a narrow amplitude range can effectively stimulate fibroblast proliferation in a relevant wound model.
    • The identified optimal parameters (37-50 mV/m at 10 Hz) fall within the range of postulated endogenous electric fields in wounds.
    • These findings support the potential therapeutic application of targeted electrical stimulation for enhancing wound healing.