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Related Experiment Videos

Characterisation of cell-surface procoagulant activities using a microcarrier model

R W Stephens1, L Orning, H Stormorken

  • 1Nycomed Pharma A/S, Oslo, Norway.

Thrombosis Research
|December 15, 1996
PubMed
Summary

This study introduces a new model to measure cell-surface procoagulant activity and inhibitors using an electromagnetic coagulometer. The model identifies tissue factor as the primary coagulation trigger on most cells, aiding in anticoagulant drug discovery.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Hematology

Background:

  • Cell surface procoagulant activity plays a critical role in hemostasis and thrombosis.
  • Characterizing these activities and their inhibitors is essential for understanding coagulation disorders and developing new therapies.

Purpose of the Study:

  • To develop and validate a novel model for characterizing cell-surface procoagulant activities and their inhibitors.
  • To investigate the procoagulant potential of various normal and transformed cell types.
  • To utilize this model as a drug discovery tool for novel anticoagulants.

Main Methods:

  • Utilized microcarrier beads to present living cells to recalcified blood plasma in an electromagnetic coagulometer.
  • Determined procoagulant activity by measuring clotting time.

Related Experiment Videos

  • Employed specifically immunodepleted plasmas and inhibitors, including monoclonal antibodies, for detailed characterization.

    • Main Results:

    • Identified tissue factor as the predominant cell surface trigger for coagulation across multiple cell types.
    • Observed direct activation of factor X independent of factor VII only in blood monocytes.
    • Demonstrated that ECV304 cell-mediated coagulation is dependent on tissue factor, factors V, VII, and X, and inhibited by specific inhibitors.

    Conclusions:

    • The novel model effectively characterizes cell-surface procoagulant activities and their inhibitors.
    • This model is valuable for studying diverse cell phenotypes and serves as a potent drug discovery tool for new anticoagulants.