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Comparable growth of a Trichomonas vaginalis strain in PEHPS and TYI-S-33 media
J Castro-Garza1, F Anaya-Velázquez, S Said-Fernández
1División de Biología Celular y Molecular, Instituto Mexicano del Seguro Social, Monterrey, Nuevo León, México. jcastro@academ01.mty.itesm.mx
Abstract:
PEHPS medium, developed for axenic cultivation of Entamoeba histolytica and E. invadens, was also capable of supporting the growth of a Trichomonas vaginalis strain, with an inoculum of 1 to 100 trichomonads/ml. The logarithmic growth phase in PEHPS or in TYI-S-33 medium lasted 72 h; yield (3.33 +/- 0.56 x 10(6) trichomonads/ml), duplication time (4.27 h), number of duplications (16.85), or increase ratio (33,328) in PEHPS medium showed no significant differences with those obtained in TYI-S-33 under similar culture conditions. Accordingly, PEHPS medium might be used for the axenic cultivation of T. vaginalis.
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