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Related Experiment Videos

Replicate plating: does it increase reliability?

K K Wille1, B R Vowels, A N Foglia

  • 1Clinical Research and Biometrics Department, Procter & Gamble Company, Cincinnati, OH, USA.

Letters in Applied Microbiology
|August 1, 1996
PubMed
Summary
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Replicate plating of microbiological specimens showed high similarity between replicate plates. Single plating is as accurate as replicate plating for evaluating erythromycin gel

Area of Science:

  • Microbiology
  • Clinical Pharmacology

Background:

  • Topical erythromycin gel is used clinically to evaluate antibacterial effects.
  • Standard microbiological procedures are essential for accurate bacterial counts.

Purpose of the Study:

  • To compare the accuracy of single versus replicate plating in a clinical study.
  • To assess the reliability of bacterial counts when using topical erythromycin gel.

Main Methods:

  • Microbiological specimens were collected from patients using erythromycin gel or placebo.
  • Specimens were plated in triplicate on bacteriological media.
  • Aerobic and anaerobic organisms were counted, and replicate plate counts were compared.

Main Results:

  • Bacterial colony counts from replicate plates demonstrated high similarity for all organism types.

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  • Statistical tests showed nearly identical results whether using single, double, or triplicate plating.
  • No significant difference in accuracy was found between single and replicate plating methods.

    • Conclusions:

    • Replicate plating does not enhance the accuracy of bacterial counts compared to single plating.
    • Single plating provides reliable bacterial counts with heightened confidence in results.
    • This finding simplifies microbiological analysis in clinical studies involving topical treatments.