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Disposable sensor for biochemical oxygen demand

Z Yang1, H Suzuki, S Sasaki

  • 1Research Center for Advanced Science and Technology, University of Tokyo, Japan.

Applied Microbiology and Biotechnology
|August 1, 1996
PubMed
Summary

New disposable microbial sensors offer a faster and more sensitive method for determining biochemical oxygen demand (BOD). These sensors utilize immobilized yeast on miniature oxygen electrodes, achieving a lower detection limit than previous technologies.

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Area of Science:

  • Environmental Science
  • Biotechnology
  • Sensor Technology

Background:

  • Biochemical oxygen demand (BOD) is a critical parameter for assessing water quality.
  • Conventional BOD determination methods are time-consuming (5-day incubation).
  • Development of rapid and sensitive BOD sensors is essential for effective wastewater management.

Purpose of the Study:

  • To develop and characterize disposable microbial sensors for rapid BOD determination.
  • To improve the sensitivity and reduce the detection limit compared to existing sensors.
  • To evaluate the sensor's performance in real-world wastewater samples.

Main Methods:

  • Immobilization of Trichosporon cutaneum yeast on miniature oxygen electrodes using UV crosslinking resin.
  • Fabrication of Clark-type two-electrode oxygen sensors on silicon substrates via micromachining.

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  • Testing sensor response time, dynamic range, and reproducibility using standard BOD solutions.

    • Main Results:

    • Sensors exhibited response times of 7-20 minutes.
    • Dynamic range of 0-18 mg/l BOD at 20°C with a lower detection limit of 0.2 mg/l.
    • Reproducibility within 8% relative standard deviation and an operational lifetime of 3 days.

    Conclusions:

    • The developed disposable microbial sensors provide a sensitive and rapid method for BOD determination.
    • Sensor performance correlated well with the conventional 5-day BOD method in analyzing industrial and municipal wastewater.
    • These sensors represent a significant advancement for on-site water quality monitoring.