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Partial interfacial activation of Proteus vulgaris lipase overexpressed in Escherichia coli
1Applied Microbiology Research Group, Korea Research Institute of Bioscience & Biotechnology, Yusung, Taejon, South Korea.
Bioscience, Biotechnology, and Biochemistry
|August 1, 1996
Abstract:
An expression plasmid carrying an alkaline lipase gene from Proteus vulgaris was constructed. The lipase content in Escherichia coli cells harboring the expression plasmid reached about 22% of total soluble protein. The purified enzyme displayed a partial interfacial activation toward p-nitrophenyl butyrate (PNPB).