Related Experiment Videos
Rapid detection and isolation of DNA-binding compounds from Streptomyces xanthochromogenes
J Holmalahti1, S Junttila, A von Wright
1Department of Pharmaceutical Chemistry, University of Kuopio, Finland.
Letters in Applied Microbiology
|October 1, 1996
Abstract:
The culture medium of Streptomyces xanthochromogenes JH903 was found to show selective activity against DNA-repair-deficient Escherichia coli CM871 strain. In this report we describe a simple method to locate and isolate DNA binding compounds from the fermentation broth. The method is based on the retention of DNA-reacting compounds in cellulose complexed with DNA, and purification of these compounds with thin-layer chromatography. Screening of microbial metabolites from chloroform extracts of fermentation media resulted in detection of five genotoxic fractions.