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Updated: Aug 15, 2026

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
Published on: March 19, 2015
Characterization of Cryptococcus neoformans by random DNA amplification
P Ruma1, S C Chen, T C Sorrell
1Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, NSW, Australia.
Abstract:
Random amplified polymorphic DNA (RAPD) was optimized and used to distinguish between the varieties and serotypes of Cryptococcus neoformans. The RAPD technique distinguished between serotypes A, D or AD within C. neoformans var. neoformans, and revealed further differentiation within each serotype. Four RAPD profiles were clearly recognizable within C. neoformans var. gattii, although its two serotypes, B and C, were only differentiated with one primer combination out of seven.
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